Ndr Protein Kinase Is Regulated by Phosphorylation on Two Conserved Sequence Motifs

Phosphorylation of cardiac protein kinase B is regulated by palmitate.

In this study isolated perfused working rat hearts were used to investigate the role of palmitate-regulated protein kinase B (PKB) phosphorylation on glucose metabolism. Rat hearts were perfused aerobically in working mode with 11 mM glucose and either 100 microU/ml insulin or 100 microU/ml insulin and 1.2 mM palmitate. PKB activity and phosphorylation state were reduced in the presence of 1.2 ...

Protein Ubiquitination Is Regulated by Phosphorylation

Protein ubiquitination has been implicated in ATPdependent protein turnover and in a number of biological processes in eukaryotic cells. The ubiquitination activating enzyme, E l , and ubiquitin carrier protein, E2, are two essential enzymes in the protein ubiquitination machinery. Using purified E l and E2 from rabbit reticulocytes and various protein kinases, which include CAMP-dependent prot...

/calmodulin-dependent Protein Kinase Kinase Is Regulated by Multi-site Phosphorylation

Mitogen-activated protein kinase kinase 1 (MKK1) is negatively regulated by threonine phosphorylation.

Mitogen-activated protein kinase kinase 1 (MKK1), a dual-specificity tyrosine/threonine protein kinase, has been shown to be phosphorylated and activated by the raf oncogene product as part of the mitogen-activated protein kinase cascade. Here we report the phosphorylation and inactivation of MKK1 by phosphorylation on threonine 286 and threonine 292. MKK1 contains a consensus phosphorylation s...

TSG101 protein steady-state level is regulated posttranslationally by an evolutionarily conserved COOH-terminal sequence.

Antisense inactivation of the tsg101 tumor susceptibility gene in murine NIH3T3 fibroblasts leads to neoplastic transformation and tumorigenesis, which are reversed by restoration of tsg101 activity. tsg101 deficiency is associated with a series of mitosis-related abnormalities, whereas overexpression of TSG101 can also result in neoplastic transformation and the perturbation of cell cycling. T...